Composite

Part:BBa_K4724028

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-10)
Revision as of 21:38, 10 October 2023 by Registry (Talk | contribs)

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IsPETaseQ119F/W159H-Linker-CBM11-6xHis Tag

IsPETase is a hydrolase produced by Ideonella sakaiensis that degrades PET. Q119F and W159H double mutants have the highest PET degradation activity among double mutants, and CBM11 is the most hydrophobic domain, so plasmids containing this composite element are constructed.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
    Illegal PstI site found at 913
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
    Illegal PstI site found at 913
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1134
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
    Illegal PstI site found at 913
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal PstI site found at 913
    Illegal AgeI site found at 546
    Illegal AgeI site found at 926
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None