Coding

Part:BBa_K4724019

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-10)
Revision as of 20:57, 10 October 2023 by Registry (Talk | contribs)

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IsPETaseS93_I94insE/W159H-6xHis Tag

IsPETase is a hydrolase produced by Ideonella sakaiensis that degrades PET. Q119F/W159H is a double mutant of IsPETase.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 793
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 549
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None