Part:BBa_K4613011

T3

This part codes for hydrophilic elastin-like polypeptides (ELPs) fused with three SpyTag sequences. With C3 , they can form polymeric scaffolds via covalent bonding between SpyTag and SpyCatcher. If you want to learn about the detailed introduction of ELPs, you can click the link below. https://parts.igem.org/Part:BBa_K4613010

Fig.1 Verification of the fabrication between T3-YFP and C3. Lane1:T3-YFP. Lane2:C3. M: Marker. Lane3: T3-YFP and C3(4° C，8h).Lane4: T3-YFP and C3(4° C，3h). Lane5: T3-YFP and C3(4° C，1h).Lane6: T3-YFP and C3(25° C，8h).Lane7: T3-YFP and C3(25° C，3h).Lane8: T3-YFP and C3(25° C，1h).Lane9: T3-YFP and C3(37° C，8h).Lane10: T3-YFP and C3(37° C，3h). Lane11: T3-YFP and C3(37° C，1h).

Reference

1. Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nature Communications,2021, 12 (1): 3422.
2. Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7.
3. Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]