Part:BBa_K4729201
NanoLuc (A. tumefaciens optimized)
Reporter genes are genetic tools that serve as vital indicators of transcriptional activity within cells. These exogenous coding regions are linked to promoter sequences and introduced into cells, allowing scientists to measure promoter activity. Once expressed, reporters are assessed by measuring either the reporter protein or its enzymatic activity, providing a direct correlation between promoter strength and reporter production. Firefly luciferase is commonly used as a bioluminescent reporter in scientific research. This enzyme is a monomer with a molecular weight of 61 kDa and was first isolated from the North American firefly, Photinus pyralis. It emits green to yellow light through a two-step reaction, falling within the 550-570 nm range of the light spectrum.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
taken from:
iGEM Tuebingen 2015
BBa_K1680009 and codon optimized for A. rhizogenes
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