Part:BBa_K4960004

pvc4

Properties

Pvc4 is a part of the sheath.

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1,Figure2)

Figure1:PVC illustrations
Figure2:Payload illustrations

The sheath in the pre-contraction state is composed of 23 stacked hexameric rings of sheath proteins, Pvc4, Pvc3, and Pvc2. Among the three, Pvc4 is slightly larger, encircles Pvc5 and forms the first layer of the sheath. Pvc4 is consisted of two major domains (domain I and II). The extra domain of Pvc4, which is smaller than the protrusion domain of Pvc3, lies right below the tail fiber, but no direct interaction is observed. The function of this small protrusion of Pvc4 also remains to be elucidated. In the assembly of the PVC particles, Pvc9 and Pvc4 attach to the initiator tube proteins.to further reinforce the baseplate consisting of Pvc8, Pvc10, Pvc11 and Pvc12.(Figure3)

Figure3.The protein pvc4 encodes Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;