RNA

Part:BBa_K4830012:Design

Designed by: Beibitkyzy Aruzhan   Group: iGEM23_NTU-Singapore   (2023-10-10)
Revision as of 19:30, 10 October 2023 by Registry (Talk | contribs)

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FANCF pegRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of FANCF pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.


Source

The original FANCF pegRNA design containing spacer and rttpbs sequence was reported in Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized those and other necessary sequences, and further combined with 65777 backbone in our lab.

References