Part:BBa_K200015

DpnII

This coding region is to be used in the composite part BBa_K200009 for the genomic deletion module of [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] by Imperial College's 2009 iGEM team.

Detail

This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37°C.

DpnII is a 4 base cutter, leaving a 4 base overhang.

The cutting site is as shown:

Usage and Biology

Enzyme Properties can be viewed at the [http://www.neb.com/nebecomm/products/productR0543.asp DpnII Datasheet] from New England Biolabs

The gene was used alongside with the restriction enzyme TaqI and the methylase Dam by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project as part of the genome deletion module.