Composite

Part:BBa_K4729707

Designed by: Yasoo Morimoto   Group: iGEM23_Marburg   (2023-10-10)
Revision as of 16:50, 10 October 2023 by Yasoo mn (Talk | contribs)

Ptac + VirG TiBo542 N80D + repABCa

Introduction

The molecular machinery of plant transformation

The problem

The virG Master-Switch construct

Constitutive expression

Inducible expression

Sodium Salicylate inhibits cell growth

Cross-talk assay

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3840
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4468
    Illegal PstI site found at 3840
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1741
    Illegal BamHI site found at 262
    Illegal BamHI site found at 1917
    Illegal BamHI site found at 2209
    Illegal XhoI site found at 227
    Illegal XhoI site found at 3480
    Illegal XhoI site found at 3834
    Illegal XhoI site found at 7176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3840
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3840
    Illegal NgoMIV site found at 111
    Illegal NgoMIV site found at 2054
    Illegal NgoMIV site found at 3894
    Illegal NgoMIV site found at 4119
    Illegal NgoMIV site found at 4224
    Illegal NgoMIV site found at 5254
    Illegal AgeI site found at 5733
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassisA. tumefaciens