Part:BBa_K4759076
petH-RBS2-petF(D21Y)-linker-GFP1-10
The P450 enzymes are redox-dependent proteins, through which they source electrons from reducing cofactors to drive their activities. This part is coding for ferredoxin reductase PetH and ferredoxin PetF from the algae (Synechocystis PCC 6803) as the redox chaperones of OleP.
Usage and Biology
After obtaining the best redox partner, we performed alanine scanning on petF to speculate which sites had a greater impact on its electron transport capacity. Finally, we found that after mutations in seven of them, the electron transport effect would change greatly, so we mutated the amino acids of these sites into other 19 amino acids by modeling and selected 23 of them to show better results.
Fig1: Fermentation of 23 mutants and control groups
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1249
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1249
Illegal NotI site found at 1022 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1249
Illegal BglII site found at 1560
Illegal BglII site found at 2229
Illegal BamHI site found at 1243 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1249
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1249
- 1000COMPATIBLE WITH RFC[1000]
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