Regulatory

Part:BBa_K4645007:Design

Designed by: Yuanpu Zhang   Group: iGEM23_HZAU-China   (2023-10-10)
Revision as of 16:12, 10 October 2023 by Zhangyuanpu (Talk | contribs) (→‎References)

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This is a promoter that is activated in an acidic environment.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a promoter that is activated in an acidic environment. It is the promoter of the acid shock protein ASP in E. coli, and its sequence contains a RstA box that can be bound by phosphorylated RstA to enhance its promoter activity in a dose-dependent manner[1].This promoter can be used to respond to changes in environmental pH and has some reference value for projects with corresponding environmental condition changes.

Source

It is the promoter of the acid shock protein ASP in E. coli. The team obtained the corresponding promoter fragment from the E. coli MG1655 genome by PCR using a high-fidelity DNA polymerase. 

References

[1]Ogasawara H, Hasegawa A, Kanda E, Miki T, Yamamoto K, Ishihama A. Genomic SELEX search for target promoters under the control of the PhoQP-RstBA signal relay cascade. J Bacteriol. 2007 Jul;189(13):4791-9.