RNA
Part:BBa_K4830000:Experience
Designed by: Beibitkyzy Aruzhan Group: iGEM23_NTU-Singapore (2023-10-09)
Revision as of 14:30, 10 October 2023 by Aruzhan001 (Talk | contribs)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4830000
The pegRNA in combination with ngRNA are used to test the efficiency of the Prime Editor containing the alternative Reverse Trancriptases, by changing nucleotide at the RNF2 target site from A to G (T to C). The plasmids containing PE, pegRNA and ngRNA were trasfected into HEK293T cells, and editing efficiency was evaluated 72hr after transfection using Sanger sequencing.
Insert the graph with different RTs.
User Reviews
UNIQ7d67d82b24deaf35-partinfo-00000000-QINU UNIQ7d67d82b24deaf35-partinfo-00000001-QINU