Composite

Part:BBa_K4724023:Design

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-10)
Revision as of 14:08, 10 October 2023 by Moxi977 (Talk | contribs) (→‎Design Notes)


IsPETase-Linker-CBM11-6xHisTag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
    Illegal PstI site found at 937
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
    Illegal PstI site found at 937
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
    Illegal PstI site found at 937
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal PstI site found at 937
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use Linker to fuse the domain to the IsPETase protein and connect 6xHis Tags behind the domain for easy purification.

Source

We use Linker to fuse the domain to the IsPETase protein and connect 6xHis Tags behind the domain for easy purification.


References