Part:BBa_K4815000
PYPH1 -> Pymaker generated yeast promoter High 1
Description: The part we provide is the functional promoter sequence (about 223 bp) generated by our AI model Pymaker. PYPH1 means it is predicted to be anti-mutant and to drive extremely high expression rate by our AI model. Origin: PYPH1 targets at s. cerevisiae (saccharomyces cerevisiae), the most studied eukaryotic expression system in synthetic biology. Loci: PYPH1 consists two parts: the core promoter and the scaffold. The core promoter is an 80 bp sequence and is seated at approximately -170 to -90 upstream to the codon(which is the presumed ). The scaffold is a preserved sequence in all PYPHs. It is a structure that we learned and utilized from a previous research that can link the core promoter with the codon and provide restriction sites of BamH I and Xho I which make it possible for the plasmids with the scaffold to be inserted by various core promoter sequences at ease. Usage: To begin with, PYPH1 can drive extremely high expression of downstream products as is predicted by our excellent AI model Pymaker. Furthermore, our experiments have abundantly validated the ability of PYPH1. Under same circumstance, PYPH1 can drive expression rate at 3 times of natural high promoters(pTEF, pGAP, pADH), which are now most widely used high expression constant promoters in enterprenurship. Meanwhile, our experiments proof that this ability of driving extremely high expression is preserved among different downstream products, which make PYPH1 an excellent and practical success. PYPH1 is anti-mutant as our excellent AI model predicts.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 198
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 78
| None |