Coding

Part:BBa_K4613168

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)
Revision as of 13:34, 10 October 2023 by Registry (Talk | contribs)

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PAL

Based on its architecture comprising Per-ARNT-Sim (PAS), AmiR and NasR transcription antitermination regulators (ANTAR) and LOV domains, the protein indentified in the gram-positive actinobacterium Nakamurella multipartita is dubbed PAL.The light-activated, sequence-specific binding of PAL to its RNA aptamer (motif 58.16, BBa_K4613453) can be principally harnessed to control bacterial expression at the mRNA level.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 235
    Illegal PstI site found at 904
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 235
    Illegal PstI site found at 904
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 235
    Illegal PstI site found at 904
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 235
    Illegal PstI site found at 904
    Illegal AgeI site found at 280
    Illegal AgeI site found at 565
    Illegal AgeI site found at 775
    Illegal AgeI site found at 886
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None