Part:BBa_K4683000
Lettuce Complement
This part is the complement sequence of the fluorescent DNA aptamer Lettuce BBa_K4245133, developed by the Weill-Cornell Medical College. Lettuce is a 99 nucleotide long DNA aptamer that induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022). This sequence can be combined with Nb.BbvCI nicking enzyme cut sites BBa_K4245006 and complementary arms to become a padlock probe for exponential Rolling Circle Amplification (eRCA) and as a reporter. Due to the eRCA process creating short sequences of DNA, with this part as the middle sequence, Lettuce aptamers can be produced and quantified (Li et al., 2017).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |