Composite

Part:BBa_K4653114:Design

Designed by: Tangkun Zhu   Group: iGEM23_SZU-China   (2023-10-10)
Revision as of 12:36, 10 October 2023 by Registry (Talk | contribs)

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pMA5 - Veg promoter - SpoVG RBS - Epr - BvEP - 2x fd terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 926
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1414
    Illegal BsaI.rc site found at 1482


Design Notes

We synthesized the nucleic acid fragment of Veg promoter-SpoVG RBS-Epr signal peptide-BvEP-T7TE Terminator and designed homologous arm primers required for seamless cloning. The plan is to link the fragment with plasmid vector pMA5 through seamless cloning, and transfer it to Bacillus subtilis WB800 to verify follow-up experiments.


Source

We synthesized the nucleic acid fragment of Veg promoter-SpoVG RBS-Epr signal peptide-BvEP-T7TE Terminator and designed homologous arm primers required for seamless cloning. The plan is to link the fragment with plasmid vector pMA5 through seamless cloning, and transfer it to Bacillus subtilis WB800 to verify follow-up experiments.

References