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Part:BBa_K4711050
Gal4(AD)+linker+Fhy1
Usage and Biology
Due to the small amount of benzo (a) pyrene in kitchen waste oil, due to its strong carcinogenic ability, it has adverse effects on the environment and human health. Therefore, we designed a related circuit to degrade benzo (a) pyrene. At the same time, in order to achieve controllability of degradation, we designed a red light switch to control the expression of degradation enzymes [1,2,6,7]. The gene circuit is shown below:
Fig 1 Benzo[a]pyrene degradation pathway
Under red light (λmax = 660 nm) or far-red light (λmax = 730 nm), PhyA reversibly changes its conformation to be able to bind to FHY1, which binds PhyA to BD (DNA binding domain) and PHY1 to AD (transcription activation domain) via linker. The principle of yeast double hybrid [12-13] was used to realize the expression of the target gene under the induction of red light.
Fig 2 Red light system schematic
Source
Potential applications
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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