Coding

Part:BBa_K4692003:Design

Designed by: Shamlan Reshamwala   Group: iGEM23_ICT-Mumbai   (2023-10-10)
Revision as of 11:40, 10 October 2023 by Shamlan (Talk | contribs) (→‎References)

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Alpha-agglutinin (SAG1) of Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 94
    Illegal AgeI site found at 682
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 786


Design Notes

The C-terminal 320 aa of SAG1 protein of Saccharomyces cerevisiae can be fused to proteins of interest to express them anchored in the yeast cell surface.


Source

This gene is from the yeast Saccharomyces cerevisiae CEN.PK.

References

https://www.yeastgenome.org/locus/S000003764