Coding

Part:BBa_K4711030

Designed by: Qingyu Wu   Group: iGEM23_OUC-China   (2023-09-22)
Revision as of 11:22, 10 October 2023 by Wuqingyu (Talk | contribs) (→‎Usage and Biology)

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Upo1

Usage and Biology

The upo1 monooxygenase is derived from M. aphidis XM01 and possesses strong benzo[a]pyrene degradation capability.According to the graph, it can be seen that after approximately 15 days, almost all of the benzopyrene has been degraded, which can demonstrate the strong benzopyrene degradation activity of the Uop1 enzyme.

Fig 1 The change in benzopyrene content

Source

M. aphidis XM01

References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 672
    Illegal PstI site found at 538
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 672
    Illegal PstI site found at 538
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
    Illegal XhoI site found at 765
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 672
    Illegal PstI site found at 538
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 672
    Illegal PstI site found at 538
    Illegal NgoMIV site found at 226
    Illegal NgoMIV site found at 976
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 385
    Illegal BsaI.rc site found at 694


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Categories
Parameters
None