Plasmid

Part:BBa_K4808011

Designed by: Zhao Guichun   Group: iGEM23_AIS-China   (2023-10-10)
Revision as of 11:08, 10 October 2023 by Sorata (Talk | contribs)

pEcCas

pEcCas plasmid carry the cas 9 protein which can cut the target gene.

Characterization

pEcCas plasmid play an important role in our CRISPR-CAS 9 knocking out experiment.

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Step1: Transformation of the pEcCas plasmid(carry cas9 protein) into E. coli AIS-0 (CICC20905). Step2: construction of the pTarget plasmid and donor DNA. Step3: transformation of pTarget plasmid and donor DNA into the AIS-0 with pEsCas inserted. Step4: Incubation of the transformed strains.


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