Part:BBa_K4605005

Used to produce fluorescent bacterial cellulose

Description

We have chosen Chromoprotein EGFP (YFP) and indigoidine as two types of dyes with different properties to enrich the pigment library. To explore whether more colored fibers could be expressed in K.xylinus, we wanted to express EGFP in K.xylinus using a strong promoter (J23100) as a way to get fluorescent cellulose membranes. Plasmids containing chromoprotein can also be used to verify the strength of the selected promoters J23104, J23100 and J23119 in K. xylinus, which would help us to build up genetic toolkit in K.xylinus and achieve the desired results when we . At the same time, we wanted to use it to viualize how cellulose wraps up K. xylinus.

Experiments

We chose to use the Anderson family promoter, first, because it is compatible with K.xylinus, and there is enough references reporting that these promoters have been tested in several of bacteria such as G. xylinus 700178, G. hansenii 53582, and K. rhaeticus iGEM.Then we selected three of the strong promoters:J23100,J23104 and J23119.We linked it to EGFP/YFP in the hope that fluorescent cellulose could be expressed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]