Coding

Part:BBa_K4965001

Designed by: Joseph Song   Group: iGEM23_RDFZ-CHINA   (2023-10-10)
Revision as of 10:43, 10 October 2023 by Registry (Talk | contribs)

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FadL+FadD

The accumulation of long chain fatty acid (LCFA) is one of the main factors leading to acne, we decided to break down LCFA. Therefore, we decided to bioengineer both FadD and FadL gene into bacteria for expression. FadL can bind to LCFA and allow it to enter the periplasmic space of EcN (the space between the cell wall and the cell membrane), while FadD can activate β-oxidation of exogenous LCFA and regulate the expression of the transcription factor FadR. The cooperative function of the two genes is believed to complete the function of metabolizing LCFA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 716
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None