Part:BBa_K4765109
Twister P1 + T7_RBS + intimin-MVN fusion + stem-loop
Introduction
We’ve developed an E. coli-cyanobacteria adhesion module by transfecting intimin-MVN fusion. Intimin-MVN fusion is composed of intimin and MVN. MVN is a lectin isolated from the cyanobacteria Microcystis aeruginosa PCC7806 and it was tested by iGEM14_Peking. Intimin which includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a beta-barrel for transmembrane insertion[1] , possesses the outer membrane anchoring of MVN. We’ve constructed this fusion protein into our ribozyme-assisted polycistronic co-expression system：pRAP.
Usage and Biology
This biological component delivers MVN to the surface of E. coli, facilitating adhesion between E. coli and Microcystis aeruginosa PCC7806. We envision that the adhesion between cyanobacteria and E. coli can promote the exchange of substances within the biofilm, enhancing the biofilm's survivability.
Characterization
Sequencing map
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| Figure1 Sequencing map of MVN
Sequencing starts from the T7 terminator, with the primer 5-GCTAGTTATTGCTCAGCGG-3.
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Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1305
Illegal SapI site found at 2058
Reference
- ↑ Piñero-Lambea, C., Bodelón, G., Fernández-Periáñez, R., Cuesta, A. M., Álvarez-Vallina, L., & Fernández, L. Á. (2015). Programming controlled adhesion of E. coli to target surfaces, cells, and tumors with synthetic adhesins. ACS Synthetic Biology, 4(4), 463–473. https://doi.org/10.1021/sb500252a
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