Coding

Part:BBa_K187027

Designed by: Julia Pon   Group: iGEM09_Alberta   (2009-10-19)
Revision as of 17:16, 19 October 2009 by JuliaPon (Talk | contribs)

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rplC, 50S ribsomal subunit, L3 in pBA

rplC encodes the 50S ribsomal subunit protein L3, an essential protein involved in initiation of 50S subunit assembly. rplC is present in plasmid pBA (part BBa_K187001), which is one of two universal plasmids for the BioBytes assembly method. For details of the BioBytes method, see its BBF RFC.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 256
    Illegal XbaI site found at 10
    Illegal PstI site found at 646
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 256
    Illegal PstI site found at 646
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 256
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 256
    Illegal XbaI site found at 10
    Illegal PstI site found at 646
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 256
    Illegal XbaI site found at 10
    Illegal PstI site found at 646
    Illegal AgeI site found at 169
    Illegal AgeI site found at 604
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None