Regulatory

Part:BBa_K4743011:Design

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-10-08)
Revision as of 09:09, 10 October 2023 by JenL (Talk | contribs) (→‎References)

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Yeast ADH1 +sqr composite promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 70


Design Notes

This part didn't been tested before. It still requires careful verification to check the functionality of this part.

Source

No

References

1.Yu, S. , Liu, J. J. , Yun, E. J. , Kwak, S. , Kim, K. H. , & Jin, Y. S. . (2018). Production of a human milk oligosaccharide 2′-fucosyllactose by metabolically engineered saccharomyces cerevisiae. Microbial Cell Factories, 17.

2.Shimizu, T., Shen, J., Fang, M., Zhang, Y., Hori, K., Trinidad, J. C., Bauer, C. E., Giedroc, D. P., & Masuda, S. (2017). Sulfide-responsive transcriptional repressor SqrR functions as a master regulator of sulfide-dependent photosynthesis. Proceedings of the National Academy of Sciences of the United States of America, 114(9), 2355–2360. https://doi.org/10.1073/pnas.1614133114