Composite

Part:BBa_K4743005:Design

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-09-16)
Revision as of 09:03, 10 October 2023 by JenL (Talk | contribs) (→‎References)

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NadV+ADH1 terminator with adapter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1689
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1689
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1689
    Illegal BglII site found at 515
    Illegal BamHI site found at 16
    Illegal BamHI site found at 367
    Illegal BamHI site found at 975
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1689
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1689
    Illegal AgeI site found at 1702
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

[1] We choose the NAMPT(NadV) from Ralstonia solanacearum, because it has the highest activity compare to other organism's NAMPT.

[2] AAGGTACCGCAGGTGGGATCC is the forward primer of Adapter 1

TCACCGGTGCAGGTGGAATTC is the reverse primer of Adapter 2

Also, BamHI is included in adapter 1 and EcoRI is included in Adpater 2. These two site can be used for cloning

Source

Ralstonia solanacearum

References

1. Martin, P. R., Shea, R. J., & Mulks, M. H. (2001). Identification of a plasmid-encoded gene from Haemophilus ducreyi which confers NAD independence. Journal of bacteriology, 183(4), 1168–1174. https://doi.org/10.1128/JB.183.4.1168-1174.2001