Part:BBa_K4743003

NadB+ADH1 terminator with adapter

It codes for L-aspartate oxidase, which is an enzyme that involved in NAD synthesis.It turns aspartate into Iminoaspartate. It is involved in the pathway: NAD biosynthesis, aspartate => quinolinate => NAD

Length checking of nadB

We use electrophoresis to check the length of the fragment

Figure 1. The length of NadB.

Overall metabolic pathway of NMN

NadB is involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.

Figure 2. The Matabolic pathway of NMN production.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1833
  Illegal XbaI site found at 702
  Illegal XbaI site found at 921
  Illegal XbaI site found at 1167
  Illegal XbaI site found at 1264
  Illegal SpeI site found at 1638
  Illegal PstI site found at 1034
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1833
  Illegal SpeI site found at 1638
  Illegal PstI site found at 1034
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1833
  Illegal BamHI site found at 16
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1833
  Illegal XbaI site found at 702
  Illegal XbaI site found at 921
  Illegal XbaI site found at 1167
  Illegal XbaI site found at 1264
  Illegal SpeI site found at 1638
  Illegal PstI site found at 1034
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1833
  Illegal XbaI site found at 702
  Illegal XbaI site found at 921
  Illegal XbaI site found at 1167
  Illegal XbaI site found at 1264
  Illegal SpeI site found at 1638
  Illegal PstI site found at 1034
  Illegal AgeI site found at 1846
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 801