Part:BBa_K4743003:Design

NadB+ADH1 terminator with adapter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1833
  Illegal XbaI site found at 702
  Illegal XbaI site found at 921
  Illegal XbaI site found at 1167
  Illegal XbaI site found at 1264
  Illegal SpeI site found at 1638
  Illegal PstI site found at 1034
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1833
  Illegal SpeI site found at 1638
  Illegal PstI site found at 1034
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1833
  Illegal BamHI site found at 16
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1833
  Illegal XbaI site found at 702
  Illegal XbaI site found at 921
  Illegal XbaI site found at 1167
  Illegal XbaI site found at 1264
  Illegal SpeI site found at 1638
  Illegal PstI site found at 1034
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1833
  Illegal XbaI site found at 702
  Illegal XbaI site found at 921
  Illegal XbaI site found at 1167
  Illegal XbaI site found at 1264
  Illegal SpeI site found at 1638
  Illegal PstI site found at 1034
  Illegal AgeI site found at 1846
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 801
  

Design Notes

No specific consider for the design

Source

Listeria innocua (serotype 6a)

References

1.Seifert, J., Kunz, N., Flachmann, R., Läufer, A., Jany, K. D., & Gassen, H. G. (1990). Expression of the E. coli nadB gene and characterization of the gene product L-aspartate oxidase. Biological chemistry Hoppe-Seyler, 371(3), 239–248.

2.Cookson, B. T., Olivera, B. M., & Roth, J. R. (1987). Genetic characterization and regulation of the nadB locus of Salmonella typhimurium. Journal of bacteriology, 169(9), 4285–4293. https://doi.org/10.1128/jb.169.9.4285-4293.1987