Composite

Part:BBa_K4613025

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)
Revision as of 08:41, 10 October 2023 by Registry (Talk | contribs)

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pQE-80L-C3

he composite part was constructed to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency. Moreover, we hope our C3, which can be found under https://parts.igem.org/Part:BBa_K4613012, can be expressed successfully in E. coli Nissle 1917 (EcN). We tried T5lac promoter from (pQE-80L).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1081
    Illegal BamHI site found at 1026
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1191
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 94


[edit]
Categories
Parameters
chassis
function