Coding

Part:BBa_K4759024

Designed by: Mengrui Tao   Group: iGEM23_Jiangnan-China   (2023-10-02)
Revision as of 08:19, 10 October 2023 by Happzly (Talk | contribs)


hemH

the insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis.


Design

Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 871
    Illegal AgeI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biosynthesis/heme
Parameters
None