Coding

Part:BBa_K4759022

Designed by: Mengrui Tao   Group: iGEM23_Jiangnan-China   (2023-10-02)
Revision as of 07:50, 10 October 2023 by Happzly (Talk | contribs)


hemC

The insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis.A gene coding for porphobilinogen deaminase (PBGD) The enzyme works by stepwise addition of pyrrolylmethyl groups until a hexapyrrole is present at the active center. The terminal tetrapyrrole is then hydrolyzed to yield the product, leaving a cysteine-bound dipyrrole on which assembly continues. H2O + 4 porphobilinogen <=> hydroxymethylbilane + 4 NH4(+)

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 262
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 262
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 262
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 262
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 874


[edit]
Categories
//function/biosynthesis/heme
Parameters
None