Coding

Part:BBa_K4711022

Designed by: Qingyu Wu   Group: iGEM23_OUC-China   (2023-09-22)
Revision as of 07:19, 10 October 2023 by Wuqingyu (Talk | contribs) (→‎References)

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SpyTag

Usage and Biology

SpyTag is a peptide that forms a spontaneous amide bond with its protein partner SpyCatcher. This protein superglue is a broadly useful tool for molecular assembly, locking together biological building blocks efficiently and irreversibly in diverse architectures.

Fig 1 SpyCatcher-SpyTag three-dimensional structure

Source

References

[1]Keeble, Anthony H ,Banerjee,et al.Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics.[J].Angewandte Chemie, 2017.DOI:10.1002/ange.201707623.

[2]Zakeri B , Fierer J O , Celik E ,et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proceedings of the National Academy of Sciences of the United States of America, 2012(12).DOI:10.1073/PNAS.1115485109. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None