Part:BBa_K4759021
hemB
the insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis. A gene coding for porphobilinogen synthase (PBGS),The enzyme catalyzes the asymmetric condensation and cyclization of two 5-aminolevulinate molecules, which is the first common step in the biosynthesis of tetrapyrrole pigments such as porphyrin, chlorophyll, vitamin B12, siroheme, phycobilin, and cofactor F430. The enzyme is widespread, being essential in organisms that carry out respiration, photosynthesis, or methanogenesis. In humans, the enzyme is a primary target for the environmental toxin Pb. The enzymes from some organisms utilize a dynamic equilibrium between architecturally distinct multimeric assemblies as a means for allosteric regulation. 2 5-aminolevulinate <=> H(+) + 2 H2O + porphobilinogen
Design
Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria
Usage and Biology
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 467
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 467
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 467
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 467
- 1000COMPATIBLE WITH RFC[1000]
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