Composite

Part:BBa_K4587194

Designed by: Yuxin Wang   Group: iGEM23_DKU   (2023-10-10)
Revision as of 07:15, 10 October 2023 by Registry (Talk | contribs)

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P_B488_05770 - RinA Amplifier Effect Module

P_B488_05770 - Amplifier Effect Module is the final version of our phage engineering genetic circuit. The B488_05770 promoter was previously identified as a specific and sensitive promoter that is under positive regulation of LdtR transcriptional factor. In this module, the activation of the B488_05770 promoter induces massive expression of RinA, the product of which further act on its downstream promoter that produces downstream effect genes, which is GFP in this case.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1511
    Illegal XhoI site found at 201
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1032
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 656


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