Coding

Part:BBa_K4692001:Design

Designed by: Shamlan Reshamwala   Group: iGEM23_ICT-Mumbai   (2023-10-10)
Revision as of 07:09, 10 October 2023 by Registry (Talk | contribs)

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Phytase from Aspergillus japonicus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 679
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence has been optimized for expression in the yeast Pichia pastoris. The native secretion signal has been replaced with the alpha mating factor sequence from Saccharomyces cerevisiae. A GS linker has been added between the alpha mating factor sequence and the coding sequence.


Source

This gene is from the filamenteous fungus Aspergillus japonicus BCC18313 (Genbank accession EU786166.1).

References