Part:BBa_K4711040

NeuAc riboswitch

Usage and biology

In order to prevent E. coli from leaking into the environment, we designed the NeuAc ribose switch. The NeuAc ribose switch consists of an RNA aptamer and a hammerhead ribozyme. The principle of its action is that NeuAc binding to the RNA aptamer causes the conformational change of the entire switch, resulting in hammerhead ribozyme self-shearing, resulting in a notch, and then under the action of ReJ ribozyme in Escherichia coli cells, the following genes are cut, thereby reducing the abundance of mRNA expressed downstream genes. In order to ensure that the switch can work effectively, there are spacers at both ends of the switch, and the 5' end is 5-AAAAATAAAAAGAAAA-3 , and the 3 'end is 5-AAAAAACAAAA-3.

Fig 1 (A) Gene circuit (B) Colony PCR (C) SDS-PAGE M: Protein Marker 1: 0mM IPTG 2: 0.2mM IPTG

Fig 1 (A) Gene circuit (B) Colony PCR (C) SDS-PAGE M: Protein Marker 1: 0mM IPTG 2: 0.2mM IPTG

References

[1]Suhyung,Cho,Bo-Rahm,et al.In vitro selection of sialic acid specific RNA aptamer and its application to the rapid sensing of sialic acid modified sugars[J].Biotechnology & Bioengineering, 2012.DOI:10.1002/bit.24737.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]