Composite

Part:BBa_K4885003:Design

Designed by: Leyu Xu   Group: iGEM23_Nanjing-SDG   (2023-09-10)
Revision as of 06:11, 10 October 2023 by Xuleyu (Talk | contribs) (→‎Source)

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Pcat1-Cat1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In C. tyrobutyricum L319, glucose is converted to pyruvate and subsequently to acetyl-CoA. Then, one part of acetyl-CoA is converted to acetate, , another part is converted to ethanol, and the rest is converted into butyryl-CoA. Butyryl-CoA is respectively converted to butyrate by butyryl-CoA/acetate CoA transferase. The CoA transferase also converts acetate back to acetyl-CoA. So CoA transferase reduces the acetyl-CoA to acetate flux and enhances the acetyl-CoA to butyrate flux. Therefore, the higher the expression of CoA transferase, the higher the butyrate/acetate ratio in the final product. The endogenous Cat1 gene of C. tyrobutyricum L319 codes the butyryl-CoA/acetate CoA transferase. Using the cat1 gene and the native Pcat1 promoter of this gene, we constructed the part of Pcat1-cat1 to overexpress cat1 in C. tyrobutyricum, in order to suppress the acetyl-CoA conversion to acetate and indirectly enhance the metabolic flux of butyrate synthesis.

Source

Pcat1 promoter sequence is from BBa_K4119011. cat1 sequence is from BBa_K4119031. terminator sequence is from BBa_K3585002.

References