DNA
stgRNA 2

Part:BBa_K4630002:Design

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-10)
Revision as of 05:37, 10 October 2023 by Registry (Talk | contribs)

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self-targeting guide RNA 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

First, we designed the SDS using our own N20 generator (see Model). Also, the sgRNA scaffold right after the SDS sequence was modified to "GGG", which gives a PAM for the knock-out. Then the part reaches self-targeting.


Source

Complete synthesis.

References