Coding

Part:BBa_K4156083:Design

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-06)
Revision as of 05:25, 10 October 2023 by Wuqingyu (Talk | contribs) (→‎Design Notes)


φ174E


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is important to note that φ174E does not exist in the igem whitelist. We have drafted a safety precaution about φ174E and submitted the check-in form to igem official. The details can be seen on our wiki.

Demonstration of the function of the lysis gene

Group: iGEM Team OUC-China 2023

Author: Jiale Hong

Summary: Successfully demonstrated the lysis function of this gene

After induction with IPTG for 2-3 hours, the lysin protein can almost completely kill all bacteria. According to the results of SDS-PAGE in the figure below, it can be observed that there are almost no protein bands in the induced group. This is because a large amount of protease is released after cell death, leading to protein degradation, indicating that the lysin protein is functioning.

Fig 1 (A) Gene circuit (B) Colony PCR (C) SDS-PAGE M: Protein Marker 1: 0mM IPTG 2: 0.2mM IPTG

Source

φ174E is a lysis gene from phage Φx174.

References