Coding

Part:BBa_K4988003

Designed by: Kunxi Liu   Group: iGEM23_Thinker-Shanghai   (2023-10-09)
Revision as of 04:53, 10 October 2023 by Registry (Talk | contribs)

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PfadBA mutant pAR+IsmA

Considering the fact that after the stimulation of oleic acid, oleic acid promoter can activate the expression of the downstream gene which ensure the stability and efficiency of the expression we decided to use oleic acid promoter as our promoter. Additionally, IsmA gene are effective in transforming and degrading cholesterol, we decided to use oleic acid to activate its function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 140
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None