Part:BBa_K4814001

RecA(K6)-hha

This is a composite part aimed to kill the bacteria after testing for DNA damage. The RecA promoter is BBa_K629001, followed by RBS (BBa_B0034), biofilm reducing protein hha (BBa_K484000), and terminator (BBa_B0015). Experiments and data shows that by using hha, after the treatment of carcinogens, the optical density of the sample decreases more significantly than the control group (E.coli top10). Detailed information of hha can be obtained from BBa_K4814000.

Figure 1 & 2. The graph of the OD600 of RecA(K6)-hha after being treated with UVB (left) and H2O2 (right).

Figure 3 & 4. The graph of the OD600 of RecA(K6)-hha after being treated with Nalidixic acid (left) and Aspartame (right).

References:

Balsalobre, C., Juárez, A., Madrid, C., Mouriño, M., Prenafeta, A., & Muñoa, F. J. (1996). Complementation of the hha mutation in Escherichia coli by the ymoA gene from Yersinia enterocolitica: dependence on the gene dosage. Microbiology (Reading, England), 142 ( Pt 7), 1841–1846. https://doi.org/10.1099/13500872-142-7-1841

Hong, S. H., Lee, J., & Wood, T. K. (2010). Engineering global regulator Hha of Escherichia coli to control biofilm dispersal. Microbial biotechnology, 3(6), 717–728. https://doi.org/10.1111/j.1751-7915.2010.00220.x

Madrid, C., Nieto, J. M., & Juárez, A. (2001). Role of the Hha/YmoA family of proteins in the thermoregulation of the expression of virulence factors. International Journal of Medical Microbiology, 291(6-7), 425-432. https://doi.org/10.1078/1438-4221-00149

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 383
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]