Coding

Part:BBa_K4672001

Designed by: Bangnuo Zhao   Group: iGEM23_SHSBNU-China   (2023-10-09)
Revision as of 03:39, 10 October 2023 by Registry (Talk | contribs)

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IntC-4XT-IntN

We designed a new part to realize polymerization of 4XT BBa_K4672000, in which we fused the C- and N-terminal of a fast-reacting SI pair to 4XT, named IntC-4XT-IntN. SIs catalyze splicing reactions spontaneously, which lead to the covalent link between fusion partners through peptide bonds to polymerize into long fibrous threads.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 701


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Parameters
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