Part:BBa_K4759001
OleP
a gene coding for multifunctional bacterial P450 monooxygenase CYP107D1(Olep), which is from the oleandomycin synthesis pathway of Streptomyces antibioticus. Cytochrome P450s (P450s) are heme-containing enzymes that catalyze various types of chemical reactions on a variety of substrates, The bacterial P450 Olep, is an epoxidase involved in the tailoring steps of the biosynthetic pathway of the antibiotic oleandomycin, OleP not only can catalyzes a regio- and stereospecific reaction of epoxidation at the C8-C8a of the macrolactone ring, but also can Regio- and stereoselective hydroxylation of bile acid such as lithocholic acid (LCA), the production can be served as potential drug candidates to treat symptoms associated with the coronavirus disease (COVID-19) infection. In our project, we found that Olep also can Regio- and stereoselective hydroxylation of deoxycholic acid (DCA) to 6β-DCA. P450s are thought to be reliable, effective, and ecofriendly biocatalysts for the synthesis of valuable compounds in recombinant hosts.
Usage and Biology
OleP, the cytochrome P450 epoxidase from Streptomyces antibioticus involved in Oleandomycin biosynthesis: functional analysis and crystallographic structure in complex with clotrimazole. PDB DOI: https://doi.org/10.2210/pdb4XE3/pdb Classification: OXIDOREDUCTASE Organism(s): Streptomyces antibioticus Expression System: Escherichia coli BL21(DE3)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 16
Illegal AgeI site found at 114 - 1000COMPATIBLE WITH RFC[1000]
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