Composite

Part:BBa_K4759203

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)
Revision as of 01:24, 10 October 2023 by JianghuaChen (Talk | contribs)


T7-RBS4-OleP

we selected high-copy plasmids pRSFDuet, medium-high copy plasmids pETDuet and pET28a, and low-copy plasmid pACYCDuet as carriers to express Olep in E. coli, and the results showed that the recombinant strains constructed with high-copy plasmid pRSFDuet as the carrier had the highest expression of p450 enzyme, cell growth rate, and substrate conversion rate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 102
    Illegal AgeI site found at 200
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None