Composite

Part:BBa_K4759202

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)
Revision as of 01:23, 10 October 2023 by JianghuaChen (Talk | contribs)


T7-RBS3-OleP

P450 enzyme CYP107D1 derived from Streptomyces, abbreviated as Olep, is selected to synthesize the above gene by adding histidine tags at the C-terminus through E. coli codon optimization and subcloning the above genes onto the plasmid pET-28a to obtain the recombinant plasmid pET-28a-oleP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal AgeI site found at 103
    Illegal AgeI site found at 201
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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