Coding
GH110b
Part:BBa_K4726002:Design
Designed by: Benjamin Ouellet Group: iGEM23_ULaval (2023-10-01)
α-galactosidase GH110b_Bacteroides fragilis
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1213
Illegal EcoRI site found at 1714 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1213
Illegal EcoRI site found at 1714 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1213
Illegal EcoRI site found at 1714 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1213
Illegal EcoRI site found at 1714 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1213
Illegal EcoRI site found at 1714 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to clone this genetic construct onto our expression vector (pET-3a) we added NdeI site in 5' and BamHI site in 3' of the gene. We added TATATA sequences before the NdeI site and after the BamHI site to assist with the restriction digestion.
Source
jbunn