Coding
P0DTR4

Part:BBa_K4726000

Designed by: Benjamin Ouellet   Group: iGEM23_ULaval   (2023-10-01)
Revision as of 00:14, 10 October 2023 by Benjamin Ouellet (Talk | contribs)


N-acetyl-α-D-galactosamine deacetylase_Flavonifractor plautii

This encodes a N-acetyl-α-D-galactosamine deacetylase from Flavonifractor plautii originating from the human gut microbiota.

This enzyme removes the acetyl moiety of N-acetyl-α-D-galactosamine (A antigen). This however does not results in full degradation of A antigen a α-D-galactosamine residue remains.

The expression of this enzyme led to significant proteotoxicity shown by an impaired growth of Escherichia coli BL21 (DE3). We thus recommended to use Escherichia coli Rosetta (DE3) pLysS which helps stabilizing pET recombinants encoding proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1894
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None