![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
P0DTR5
Part:BBa_K4726001:Design
Designed by: Benjamin Ouellet Group: iGEM23_ULaval (2023-10-01)
α-D-galactosamine galactosaminidase_Flavonifractor plautii
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2998
Illegal PstI site found at 77
Illegal PstI site found at 571 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2998
Illegal PstI site found at 77
Illegal PstI site found at 571 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2998
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2998
Illegal PstI site found at 77
Illegal PstI site found at 571 - 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to clone this genetic construct onto our expression vector (pET-3a) we added NdeI site in 5' and BamHI site in 3' of the gene. We added TATATA sequences before the NdeI site and after the BamHI site to assist with the restriction digestion.
Source
This gene was originally taken from the Flavonifractor plautii bacteria. It encodes a α-D-galactosamine galactosaminidase.
The amino acids sequence encoded by this gene is: