Composite

Part:BBa_K4632024

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-09)
Revision as of 21:38, 9 October 2023 by Registry (Talk | contribs)

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Quorum sensing-based T4 lysis device

This part is a Quorum Sensing-based T4 lysis device constructed based on the LasI-LasR system (QS-2) and the T4-T4 lysis device.

The QS-2 system is a Quorum Sensing system from 'Pseudomonas aeruginosa.' As modified by Wei Jiang et al., it can be entirely orthogonal to the TraI-TraR system (QS-1), another Quorum Sensing system from 'Agrobacterium tumefaciens' (Jiang et al., 2020).

To achieve control over toxicant concentration, we introduced components labeled with BBa_K4632016 and BBa_K4632016 to create the T4-T4 lysis device.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 84
    Illegal XbaI site found at 795
    Illegal PstI site found at 367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 367
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 84
    Illegal XbaI site found at 795
    Illegal PstI site found at 367
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 84
    Illegal XbaI site found at 795
    Illegal PstI site found at 367
    Illegal AgeI site found at 1089
    Illegal AgeI site found at 1159
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None