Part:BBa_K216004

Trz hybrid signal transduction protein

Trz hybrid signal transduction protein: this was created by fusing the extracellular receptor domain of the chemoreceptor Trg with the intracellular signal transduction domain of 2-component sensor protein EnvZ, which normally phosphorylates response regulator OmpR (see Baumgartner, J.W., Kim, C., Brissette, R.E., Inouye, M., Park, C., and Hazelbauer, G.L. 1994. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of the osmosensor EnvZ. J. Bacteriol. 176, 1157-1163). This part can activate genes attached to an OmpR-responsive promoter such as PompC. To avoid inappropriate activation via EnvZ, it may be necessary to use a chassis in which EnvZ is not present.

Usage and Biology

Trz is a fusion protein with the extracellular region of the chemoreceptor Trg fused to the intracellular kinase domain of two-component sensor EnvZ, an osmosensor which phosphorylates the cognate response regulator OmpR. Phosphorylated OmpR then activates promoters such as the ompC promoter (available as BBa_R0082). Trg normally detects chemoattractants such as ribose, bound to the periplasmic ribose-binding protein. The usefulness of Trz arises in that ribose binding protein can be computationally redesigned to bind non-natural ligands, and this binding can then be detected via actiavtion of a reporter gene fused to the ompC promoter (Looger et al, 2003). Thus this is potentially a generalisable transduction system for detection of extracellular ligands.

Trz was previously deposited as part (...) by (...); however, Registry sequence analysis of this part indicates that the sequence is not correct. It appears in fact to be ... This part is therefore a replacement for ... We have confirmed the sequence of our part but at the time of writing have not yet confirmed its activity.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 365
  
• 1000
  COMPATIBLE WITH RFC[1000]